Immunology, Transplantation and Infectious diseases
Dynamics of immune responses
This group seeks to dissect the complex dynamics of host-virus interactions with a particular focus on the development and function of adaptive immune responses. Since it is still beyond the reach of even the most sophisticated in vitro methodology to simulate the complex interplay of physical, cellular, biochemical, and other factors that influence cell behavior in microvessels and interstitial tissues, we make use of intravital microscopy. This technique is complemented by more traditional molecular, cellular and histological approaches, thus characterizing host-virus interactions at the molecular, single cell- and whole animal-level.
Fumagalli V, Venzin V, Di Lucia P, Moalli F, Ficht X, Ambrosi G, Giustini L, Andreata F, Grillo M, Magini D, Ravà M, Friedrich C, Fontenot JD, Bousso P, Gasteiger G, Vivier E, Kuka M, Guidotti LG, Iannacone M Group 1 ILCs regulate T cell-mediated liver immunopathology by controlling local IL-2 availability. Science Immunology, 2022 7:eabi6112
Fumagalli V, Ravà M, Marotta D, Di Lucia P, Laura C, Sala E, Grillo M, Bono E, Giustini L, Perucchini C, Mainetti M, Sessa A, Garcia-Manteiga JM, Donnici L, Manganaro L, Delbue S, Broccoli V, De Francesco R, D’Adamo P, Kuka M, Guidotti LG, Iannacone M Administration of aerosolized SARS-CoV-2 to K18-hACE2 mice uncouples respiratory infection from fatal neuroinvasion. Science Immunology, 2022, 7:eabl9929
De Simone G, Andreata F, Bleriot C, Fumagalli V, Laura C, Garcia-Manteiga JM, Di Lucia P, Gilotto S, Ficht X, Bono EB, Giustini L, De Ponti F, Ambrosi G, Mainetti M, Zordan P, Benechet AP, Ravà M, Chakarov S, Moalli F, Bajenoff M, Guidotti LG, Ginhoux F, Iannacone M Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming. Immunity, 2021 54:2089
Fumagalli V, Di Lucia P, Venzin V, Bono E, Jordan R, Frey C, Delaney W, Chisari FV, Guidotti LG, Iannacone M Antibody-mediated clearance of serum HBsAg has minimal impact on CD8+ T cell responses in mouse models of HBV pathogenesis. Journal of Experimental Medicine, 2020 217:e20200298
De Giovanni M, Cutillo V, Giladi A, Maganuco CG, Medaglia C, Bono E, Di Lucia P, Giustini L, Cristofani C, Pinschewer DD, Kastenmuller W, Amit I, Kuka M, Iannacone M Spatiotemporal regulation of type I interferon expression determines antiviral CD4+ T cell polarization. Nature Immunology, 2020, 21:321
Benechet AP*, De Simone G*, Di Lucia P, Cilenti F, Barbiera G, Le Bert N, Moalli F, Lusito E, Fumagalli V, Bianchessi V, Zordan P, Bono E, Giustini L, Bonilla WD, Bleriot C, Kunasegaran K, Gonzalez- Aseguinolaza G, Pinschewer DD, Kennedy PTF, Naldini L, Kuka M, Ginhoux F, Cantore A, Bertoletti A, Ostuni R#, Guidotti LG#, Iannacone M#, Dynamics and genomic landscape of CD8+ T cells undergoing hepatic priming. Nature, 2019, 574:200 (*co-first authors; #co-last authors)
Medaglia C*, Giladi A*, Stoler Barak L*, De Giovanni M*, Meir Salame T, Biram A, David E, Iannacone M#, Shulman Z#, Amit I#, Spatial reconstruction of immune niches by combining photoactivatable fluorescent reporters and single-cell RNA-seq, Science, 2017, 358:1622 (*co-first authors; #co-last and co-corresponding authors)
Jimenez de Oya N, De Giovanni M, Fioravanti J, Ubelhart R, Di Lucia P, Fiocchi A, Iacovelli S, Efremov DG, Caligaris-Cappio F, Jumaa H, Ghia P, Guidotti LG, Iannacone M Pathogen-specific B cell receptors drive chronic lymphocytic leukemia by light chain-dependent cross-reaction with autoantigens. EMBO Molecular Medicine, 2017, 9:1482
Fioravanti J, Magini D, Di Lucia P, Boni C, Benechet AP, Inverso D, Fiocchi A, Wieland S, Purcell R, Ferrari C, Chisari FV, Guidotti LG, Iannacone M Effector CD8+ T cell-derived interleukin-10 enhances liver immunopathology. Journal of Hepatology, 2017, 67:543
Sammicheli S*, Kuka M*, Di Lucia P, Jimenez de Oya N, De Giovanni M, Fioravanti J, Cristofani C, Maganuco CG, Fallet B, Ganzer L, Sironi L, Mainetti M, Ostuni R, Larimore K, Greenberg PD, de la Torre JC, Guidotti LG, Iannacone M, Inflammatory monocytes hinder antiviral B cell responses. Science Immunology, 2016, 1:eaah6789 (*co-first authors)
Guidotti LG*, Inverso D*, Sironi L, Di Lucia P, Fioravanti J, Fiocchi A, Vacca M, Aiolfi R, Sammicheli S, Mainetti M, Ganzer L, Cataudella T, Raimondi A, Gonzalez-Aseguinolaza G, Protzer U, Ruggeri ZM, Chisari FV, Isogawa M, Sitia G, Iannacone M, Immunosurveillance of the liver by intravascular effector CD8+ T cells, Cell, 2015, 161:486 (*co-first authors)
Tonti E, Jimenez de Oya N, Galliverti G, Moseman EA, Di Lucia P, Amabile A, Sammicheli S, De Giovanni M, Sironi L, Chevrier N, Sitia G, Gennari L, Guidotti LG, von Andrian UH, Iannacone M Bisphosphonates target B cells to enhance humoral immune responses. Cell Reports, 2013, 5:323
Iannacone M*✉, Moseman EA*, Tonti E, Bosurgi L, Henrickson SE, Whelan S, Guidotti LG, von Andrian UH, Subcapsular sinus macrophages prevent CNS invasion on peripheral infection with a neurotropic virus, Nature, 2010, 465:1079 (*co-first authors)
Iannacone M, Sitia G, Isogawa M, Whitmire JK, Marchese P, Chisari FV, Ruggeri ZM, Guidotti LG, Platelets prevent IFN-ab-induced lethal hemorrhage promoting CTL-dependent clearance of lymphocytic choriomeningitis virus. PNAS, 2008, 105:631
Iannacone M, Sitia G, Isogawa M, Marchese P, Castro MG, Lowenstein PR, Chisari FV, Ruggeri ZM, Guidotti LG, Platelets mediate cytotoxic T lymphocytes-induced liver damage. Nature Medicine, 2005, 11:1167
Marta Grillo
PhD student
Chiara Perucchini
Technician
Francesco Andreata
Postdoc fellow
Keigo Kawashima
Postdoc fellow
Elisa Bono
Technician
Caroline Claire Kruger
Postdoc fellow
Elena Rodriguez Bovolenta
Postdoc fellow
Pietro Di Lucia
Lab manager, Immunopathology Unit
Mattia Freschi
Technician
Valeria Fumagalli
Postdoc fellow
Leonardo Giustini
Technician
Federica Moalli
Postdoc fellow
Mirela Kuka
Assistant professor
Marta Mainetti
Technician, Immunopathology Unit
Cristian Beccaria
Postdoc fellow
Eleonora Sala
PhD student
Xenia Ficht
Postdoc fellow
Micol Ravà
Postdoc fellow
Chiara Laura
PhD student
Davide Marotta
PhD student
Valentina Venzin
PhD student