Experimental Imaging Center

Correlative Light Electron Microscopy



Andrea Raimondi


Correlative Light Electron Microscopy (CLEM) is a powerful approach that can combine the capabilities of light and electron microscopy imaging techniques overcoming their intrinsic limitations.

We are seeking to optimize and develop new CLEM approaches in order to be able to analyze at ultrastructural level also rare and unique events. So far we established several protocols that let us to identify at EM level the cell of interest in a contest of a tissue or localize with high spatial resolution a fluorescent signal by means of DAB photo-oxidation.