Experimental Imaging Center
Correlative Light Electron Microscopy
Correlative Light Electron Microscopy (CLEM) is a powerful approach that can combine the capabilities of light and electron microscopy imaging techniques overcoming their intrinsic limitations.
We are seeking to optimize and develop new CLEM approaches in order to be able to analyze at ultrastructural level also rare and unique events. So far we established several protocols that let us to identify at EM level the cell of interest in a contest of a tissue or localize with high spatial resolution a fluorescent signal by means of DAB photo-oxidation.
Guarnieri FC, Pozzi D, Raimondi A, Fesce R, Valente MM, Delvecchio VS, Van Esch H, Matteoli M, Benfenati F, D'Adamo P, Valtorta F. A novel SYN1 missense mutation in non-syndromic X-linked intellectual disability affects synaptic vesicle life cycle, clustering and mobility. Hum Mol Genet. 2017 Sep 11. doi: 10.1093/hmg/ddx352
Bononi A, Giorgi C, Patergnani S, Larson D, Verbruggen K, Tanji M, Pellegrini L, Signorato V, Olivetto F, Pastorino S, Nasu M, Napolitano A, Gaudino G, Morris P, Sakamoto G, Ferris LK, Danese A, Raimondi A, Tacchetti C, Kuchay S, Pass HI, Affar EB, Yang H, Pinton P, Carbone M. BAP1 regulates IP3R3-mediated Ca(2+) flux to mitochondria suppressing cell transformation. Nature. 2017 Jun 22;546(7659):549-553
Caldieri G, Barbieri E, Nappo G, Raimondi A, Bonora M, Conte A, Verhoef LGGC, Confalonieri S, Malabarba MG, Bianchi F, Cuomo A, Bonaldi T, Martini E, Mazza D, Pinton P, Tacchetti C, Polo S, Di Fiore PP, Sigismund S. Reticulon 3-dependent ER-PM contact sites control EGFR nonclathrin endocytosis. Science. 2017 May 12;356(6338):617-624
Fumagalli F, Noack J, Bergmann TJ, Cebollero E, Pisoni GB, Fasana E, Fregno I, Galli C, Loi M, Soldà T, D'Antuono R, Raimondi A, Jung M, Melnyk A, Schorr S, Schreiber A, Simonelli L, Varani L, Wilson-Zbinden C, Zerbe O, Hofmann K, Peter M, Quadroni M, Zimmermann R, Molinari M. Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery. Nat Cell Biol. 2016 Nov;18(11):1173-1184
Guidotti LG, Inverso D, Sironi L, Di Lucia P, Fioravanti J, Ganzer L, Fiocchi A, Vacca M, Aiolfi R, Sammicheli S, Mainetti M, Cataudella T, Raimondi A, Gonzalez-Aseguinolaza G, Protzer U, Ruggeri ZM, Chisari FV, Isogawa M, Sitia G, Iannacone M. Immunosurveillance of the liver by intravascular effector CD8(+) T cells. Cell. 2015 Apr 23;161(3):486-500
Lignani G, Raimondi A, Ferrea E, Rocchi A, Paonessa F, Cesca F, Orlando M, Tkatch T, Valtorta F, Cossette P, Baldelli P, Benfenati F. Epileptogenic q555x syn1 mutant triggers imbalances in release dynamics and short-term plasticity. Hum Mol Genet. 2013; 22(11):2186-99
Raimondi A, Ferguson S; Lou X; Armbruster M; Paradise S; Giovedi S; Messa M; Kono N; Takasaki J; Capello V; O'Toole E; Ryan T; De Camilli P. Overlapping role of dynamin isoforms in synaptic vesicle endocytosis. Neuron. 2011; 70(6):1100-14
Hayash M, Raimondi A, O'TooleE, Paradise S, Collesi C .Cremona O, Ferguson S.M, De Camilli P. Cell and stimulus dependent heterogeneity of synaptic vesicle endocytic recycling revealed by studies of dynamin 1-null neurons. Proc. Nat. Acad. Sci. 2008; 105(6):2175-80
Ferguson S.M, Brasnjo G, Hayashi M, Wolfel M, Collesi C, Giovedi S, Raimondi A, Gong L, Ariel P, Paradise S, O’Toole E, Flavell R, Cremona O, Miesenbock G, Ryan T.A, De Camilli P. A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science. 2007; 316:511-3
Raimondi A, Mangolini A, Rizzardini M, Tartari S, Bendotti C, Borgese N, Francolini M, Cantoni L, Pietrini G. Cell culture models to investigate the selective vulnerability of motoneuronal mitochondria to familial ALS-linked G93A SOD1. European Journal of Neuroscience. 2006; 24:387-99