Jess - Chemiluminescent and Fluorescent capillary Western Blotting (Proteinsimple)
The JESS capillary Western blotting is a technology for automated protein separation and immunodetection equivalent to those obtainable with traditional Western Blot, but with a number of advantages. The system exploits a technology with fully automated capillary microfluidics that provides for immobilization of the antigen directly in the capillaries. The electrophoretic run, the interaction with the antibody, as well as the quantification of the signal takes place in the capillaries. JESS separates and analyzes proteins by size from 2–440 kDa either by immunoassay, using chemiluminescent or fluorescent probes, or total protein analysis. The amount of both antigen and antibody required is extremely reduced low (protein: 0.5-4 ug/3uL/well), and both, multiplexing and protein quantification, are possible. Compared to the classical Western blot method, JESS allows to complete an experiment in 3-4 hours and with minimal operator intervention.
The instrument does not intend to replace the classical method, but is added to it for experiments for which there is little starting material or in case of needs of high sensitivity. A practical advantage for "publication" experiments is that the final result is entirely digital, thus, independently from the loading position, the result can be presented with an image that follows the classic Western blot presentation with the samples in the order you like more.