The in vitro fertilization assay is an assisted reproduction technique that is used to rescue a murine line that presents reproductive obstacles and revitalize a frozen line starting by cryopreserved sperms.

Fresh or frozen sperm is incubated with donor eggs, after 5 hours, embryos are washed, transferred into fresh medium and leaved to reach the 2-cell developmental stage. All embryos are then transferred into foster mothers.

What CFCM staff needs:

• A male mouse heterozygous or homozygous for the mutation;
• 2 straws of frozen sperm.