Sperm freezing allows murine lines preservation, protects mice from contaminations in the animal house and lab accidents. Moreover, it allows conservation of murine lines not necessary for current studies. The consequence is the saving of precious space in the animal house and cage costs.
CFCM recovers sperm from two mutated mice and their semen is frozen into 20 straw, 10 per each male. After sperm freezing, two quality tests are available to the customer:
- In vitro sperm quality test: a straw per each line will be thawed for in vitro fertilization assay (IVF).
- In vivo sperm quality test: a straw per each line will be thawed for in vitro fertilization assay (IVF) followed by transfer of developed embryos into a foster mother.
Researchers receive caudal biopsies for genotyping.
What CFCM staff needs:
- 2 proven breeder males heterozygous or homozygous for the mutation (2-4 months old)