description

The Amnis ImageStream 100 from Amnis (now part of MERK - Millipore) is the first commercially available imaging flow cytometer. It combines advantages of flow cytometry (in terms of ability to detect cells in suspension, speed, sensitivity with a large number of sampling cells) with those of microscopy analysis (quantitative and qualitative features, localization of fluorescent probes, image analysis-based methods). Precisely-controlled fluidics positions cells in the plane of focus as they flow through the system smoothly, without tumbling and a patented velocity detection system synchronizes the CCD camera readout with the motion of the cells. T

his Instrument is provided by a brightfield lamp and 405, 488, and 658 nm laserlines. The images include brightfield (transmitted light), 488nm side-scatter, and 4 emitted fluorescence channels corresponding to those used on many 4-color flow cytometers. Cellular imagery is split into six component images through a unique spectral decomposition element. The result is a brightfield image, a darkfield image and multiple fluorescence images of every cell. The ImageStream 100 is also provided with an Extended Depth of Field (EDF), a combination of specialized optics and unique image processing algorithms that project all structures and probes within the cell into one crisply-focused image.

The IDEAS software of ImageStream, can analyse a large number of images, providing hundreds of features for every cell. IDEAS integrates image data, plots and statistics: the gallery shows images of every cell and the tabular data section allows you to view population statistics as well as individual feature sets.