description

The ImageStream MKII from Amnis (now part of CytekBio) is the first commercially available imaging flow cytometer. It combines advantages of flow cytometry (in terms of ability to detect cells in suspension, speed, sensitivity with a large number of sampling cells) with those of microscopy analysis (quantitative and qualitative features, localization of fluorescent probes, image analysis-based methods). Precisely-controlled fluidics positions cells in the plane of focus as they flow through the system smoothly, without tumbling and a patented velocity detection system synchronizes the CCD camera readout with the motion of the cells.

This Instrument is provided by a brightfield lamp and 405, 488, and 642 nm laserlines. The images include brightfield (transmitted light), 488nm side-scatter, and 4 emitted fluorescence channels corresponding to those used on many 4-color flow cytometers. Cellular imagery is split into six component images through a unique spectral decomposition element. The result is a brightfield image, a darkfield image and multiple fluorescence images of every cell.

The IDEAS software of ImageStream, can analyze a large number of images, providing hundreds of features for every cell. IDEAS integrates image data, plots and statistics: the gallery shows images of every cell and the tabular data section allows you to view population statistics as well as individual feature sets.