ALEMBIC
ImageStream
Merck Amnis ImageStreamX
Objectives
- UplanFI 4 0X (NA 0.7 5 ) Dry
Laserlines
- 405 nm , 48 8 nm , 658 nm (DPSS)
Specifics
- Brightfield (contrast): yes (Brightfield and darkfield);
- Fluor: yes;
- Sensor: CCD Camera;
- Speed: Up to 100 cells.
- Time lapse: no;
- ROI Bleaching: no;
- Other: Non-adherent cells imaging.
description
The Amnis ImageStream 100 from Amnis (now part of MERK - Millipore) is the first commercially available imaging flow cytometer. It combines advantages of flow cytometry (in terms of ability to detect cells in suspension, speed, sensitivity with a large number of sampling cells) with those of microscopy analysis (quantitative and qualitative features, localization of fluorescent probes, image analysis-based methods). Precisely-controlled fluidics positions cells in the plane of focus as they flow through the system smoothly, without tumbling and a patented velocity detection system synchronizes the CCD camera readout with the motion of the cells. T
his Instrument is provided by a brightfield lamp and 405, 488, and 658 nm laserlines. The images include brightfield (transmitted light), 488nm side-scatter, and 4 emitted fluorescence channels corresponding to those used on many 4-color flow cytometers. Cellular imagery is split into six component images through a unique spectral decomposition element. The result is a brightfield image, a darkfield image and multiple fluorescence images of every cell. The ImageStream 100 is also provided with an Extended Depth of Field (EDF), a combination of specialized optics and unique image processing algorithms that project all structures and probes within the cell into one crisply-focused image.
The IDEAS software of ImageStream, can analyse a large number of images, providing hundreds of features for every cell. IDEAS integrates image data, plots and statistics: the gallery shows images of every cell and the tabular data section allows you to view population statistics as well as individual feature sets.
Price-list (€ per hour) |
Outside Users |
In-House (San Raffaele) Users |
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Profit |
Non-Profit |
Profit |
Non-Profit |
Practical training* |
70,00 |
42,00 |
42,00 |
21,00 |
Relative Charge |
100% |
60% |
60% |
27% |
|
72,00 |
44,00 |
44,00 |
19,00 ** |
*Up to 4 persons per group. Each person is fully charged for the total number of hours of personal training, independently of the number of participants. No additional fees are charged for the use of the instruments.
**From 8 pm to 8 am or during weekends/holyday: 13,00
Price-list (€ per hour) |
Outside Users |
In-House (San Raffaele) Users |
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|
Profit |
Non-Profit |
Profit |
Non-Profit |
|
142,00 |
86,00 |
86,00 |
38,00 |
Price-list (€ per hour) |
Outside Users |
In-House (San Raffaele) Users |
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Profit |
Non-Profit |
Profit |
Non-Profit |
Specime Preparation |
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Imm. fluor. |
80,00 |
48,00 |
48,00 |
22,00 |
Data Analysis |
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80,00 |
48,00 |
48,00 |
22,00 |
Consumable |
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as per use |
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