ALEMBIC
Leica SP2 Confocal
Leica TCS SP2 Laser Scanning Confocal
Objectives
- HC PL APO CS 10X (NA 0.4) Imm.
- HC PL APO CS 20X (NA 0.7) Imm.
- HCX PL APO CS 40X (NA 1.25) Oil
- HCX PL APO CS 63X (NA 1.4) Oil
- HCX PL APO CS 63X (NA 1.2) Water
Laserlines
- 405 nm (Diode)
- 458 nm, 476 nm, 488 nm, 514 nm (Ar)
- 543 nm (HeNe)
- 633 nm (HeNe)
Specifics
- Brightfield (contrast): yes;
- Fluor: yes;
- Sensor: Tunable PMT with AOBS;
- Max resolution: 4096x4096;
- Z section: yes;
- Time lapse: yes;
- ROI Bleaching: yes;
- Other: Incubator (37°) / Spectral analysis.
description
Leica TCS SP2 is a laser scanning confocal microscope with electronically controlled and freely definable AOBS (Acousto - Optical Beam Splitter). This element replaces the conventional primary dichroic mirror in the scan head for reflecting excitation light and transmitting emitted fluorescent light. With the AOBS, a freely programmable and highly efficient single or multi-chroic beam splitter has been achieved. Highly sensitive detectors are optimized for different spectral bands.
Leica Confocal Software (LCS) complements the optics and electronics by providing a user-configurable interface, easy to use for the occasional user yet powerful to control the most advanced experiments. In addition to multi-dimensional control & analysis, advanced multi-color restoration and spectral un-mixing software allow the efficient elimination of residual crosstalk between spectrally over lapping dyes.
Leica TCS SP2 spectral confocal system provides a spectral scanning range from UV to IR. Investigators can use a large variety of fluorescent probes.
This system is equipped by microscope cage incubator in order to maintain 37° C cell col ture all around microscopy workstation.
Download the hands-on guide
Price-list (€ per hour) |
Outside Users |
In-House (San Raffaele) Users |
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Profit |
Non-Profit |
Profit |
Non-Profit |
Practical training* |
70,00 |
42,00 |
42,00 |
21,00 |
Relative Charge |
100% |
60% |
60% |
27% |
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60,00 |
36,00 |
36,00 |
16,00** |
*Up to 4 persons per group. Each person is fully charged for the total number of hours of personal training, independently of the number of participants. No additional fees are charged for the use of the instruments.
**From 8 pm to 8 am or during weekends/holyday: 12,00
Price-list (€ per hour) |
Outside Users |
In-House (San Raffaele) Users |
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|
Profit |
Non-Profit |
Profit |
Non-Profit |
|
130,00 |
78,00 |
78,00 |
35,00 |
Price-list (€ per hour) |
Outside Users |
In-House (San Raffaele) Users |
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Profit |
Non-Profit |
Profit |
Non-Profit |
Specime Preparation |
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Criostate |
80,00 |
48,00 |
48,00 |
22,00 |
Imm. fluor. |
80,00 |
48,00 |
48,00 |
22,00 |
Data Analysis |
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80,00 |
48,00 |
48,00 |
22,00 |
Consumable |
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as per use |
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