Leica SP5 Confocal

Leica TCS SP5 Laser Scanning Confocal


  • HC PL FLUOTAR 10X (NA 0.3) Dry
  • HC PL APO CS 20X (NA 0.7) Dry
  • HCX PL APO λ blue 40X (NA 1.25) Oil
  • HCX PL APO λ blue 63X (NA 1.4) Oil
  • 405 nm (Diode)
  • 458 nm, 476 nm, 488 nm, 496 nm, 514 nm (Ar)
  • 543 nm (HeNe)
  • 633 nm (HeNe)
  • Brightfield (contrast): yes;
  • Fluor: yes;
  • Sensor: Tunable PMT with AOBS;
  • Max resolution: 4096x4096;
  • Z section: yes;
  • Time lapse: yes;
  • ROI Bleaching: yes;
  • Other: Spectral analysis.


The Leica TCS SP5 Confocal covers a broad range of requirements in confocal imaging with the full array of scan speeds at highest resolution. With its high-efficiency SP detection (five channels simultaneously) and the AOBS (Acousto - Optical Beam Splitter), the system delivers bright, noise-free images with minimal photo damage at high speed and with highly sensitive detectors optimized for different spectral bands.

The Leica Application Suite Advanced Fluorescence (LASAF) complements the optics and electronics by providing a user-configurable interface, easy to use for the occasional user yet powerful to control the most advanced experiments. In addition to multi-dimensional control & analysis, advanced multi-color restoration and spectral un-mixing software allow the efficient elimination of residual crosstalk between spectrally overlapping dyes.

The Leica TCS SP5 spectral confocal system provides a spectral scanning range from UV to IR. Investigators can use a large variety of fluorescent probes.

Download the hands-on guide