ALEMBIC
TEM FEI Tecnai
FEI Tecnai G2 20 Transmission Electron Microscope
- Voltage Max: LaB6 120/200 kV;
- Sensor: CCD Camera (proscan camera);
- Max resolution: 2048x2048
- Others: Freeze Substitution; High Pressure Freezing; 3D Tomography; CLEM.
Ospedale San Raffaele has become a partner of the Center for Nanomedicine (CEN). Three of the members of CEN, i.e. Fondazione Filarete, IFOM-IEO Campus and Ospedale San Raffaele, have created a common Department of Ultrastructural and Molecular Imaging, with the mission of creating, hosting and nurturing high-tech imaging entrepreneurial initiatives in the field of biopharmacology and biomedicine. The newly formed Department has recently acquired state of the art technologies concerning EM sample preparation and imaging.
Thanks to these technologies now available, ALEMBIC will offer to San Raffaele scientists new EM methodologies, namely High Pressure Freezing and Freeze substitution, 3D Electron Tomography and CLEM (Correlative Light Electron Microscopy). Please contact the ALEMBIC Staff if you are interested in.
FEI Tecnai G2 20 charectistics: LaB6 120/200 kV; Twin lens; STEM System with HAADF detector; Tomography Holder; Gatan Cryo-transfer and Cryo-holder; Eagle 2k CCD camera.
Price-list (€ per hour) |
Outside Users |
In-House (San Raffaele) Users |
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Profit |
Non-Profit |
Profit |
Non-Profit |
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220,00 |
130,00 |
130,00 |
59,00 |
Price-list (€ per hour) |
Outside Users |
In-House (San Raffaele) Users |
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|
Profit |
Non-Profit |
Profit |
Non-Profit |
EIC/CIS |
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Negative staining |
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Preparation |
50,00 |
30,00 |
30,00 |
14,00 |
9,00 | |
Immunogold |
240,00 |
145,00 |
145,00 |
65,00 |
43,00 | |
Tot. |
290,00 |
175,00 |
175,00 |
79,00 |
52,00 | |
Processing of Epon Sample |
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|
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Immunogold pre-embedding |
240,00 |
145,00 |
145,00 |
65,00 |
43,00 | |
Preparation |
190,00 |
115,00 |
115,00 |
51,00 |
34,00 | |
Sample cut sectioning (5 grids) |
380,00 |
220,00 |
220,00 |
103,00 |
68,00 | |
Tot. |
810,00 |
480,00 |
480,00 |
219,00 |
145,00 | |
Processing of LRW Sample |
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|
|
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Preparation |
190,00 |
115,00 |
115,00 |
51,00 |
34,00 | |
Sample cut sectioning (5 grids) |
520,00 |
310,00 |
310,00 |
140,00 |
94,00 | |
Immunogold |
240,00 |
145,00 |
145,00 |
65,00 |
43,00 | |
Tot. |
950,00 |
570,00 |
570,00 |
256,00 |
171,00 | |
Processing of frozen Sample |
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|
|
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Preparation |
280,00 |
170,00 |
170,00 |
76,00 |
50,00 | |
Sample cut sectioning (5 grids) |
580,00 |
350,00 |
350,00 |
157,00 |
104,00 | |
Immunogold |
480,00 |
290,00 |
290,00 |
130,00 |
86,00 | |
Tot. |
1340,00 |
810,00 |
810,00 |
363,00 |
240,00 | |
Processing of high pressure frozen Sample (Epon) |
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Preparation (up to 10 pcs) |
250,00 |
150,00 |
150,00 |
68,00 |
45,00 | |
Freeze substitution (up tp 10 pcs) |
350,00 |
210,00 |
210,00 |
95,00 |
63,00 | |
Sample cut sectioning (5 grids) |
380,00 |
220,00 |
220,00 |
103,00 |
68,00 | |
Tot. |
980,00 |
580,00 |
580,00 |
266,00 |
176,00 | |
Processing of high pressure frozen Sample (Acrylic) |
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|
|
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Preparation (up to 10 pcs) |
250,00 |
150,00 |
150,00 |
68,00 |
45,00 | |
Freeze substitution (up to 10 pcs) |
350,00 |
210,00 |
210,00 |
95,00 |
63,00 | |
Sample cut sectioning (5 grids) |
520,00 |
310,00 |
310,00 |
140,00 |
94,00 | |
Immunogold |
240,00 |
145,00 |
145,00 |
65,00 |
43,00 | |
Tot. |
1360,00 |
815,00 |
815,00 |
368,00 |
245,00 | |
3D Tomography |
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|
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Titl series acquisition, Tomographic reconstruction |
680,00 |
400,00 |
400,00 |
184,00 |
122,00 | |
Tomogram segmentation and 3D rendering (per hour) |
100,00 |
60,00 |
60,00 |
27,00 |
18,00 | |
Data analysis (per hour) |
100,00 |
60,00 |
60,00 |
27,00 |
18,00 | |
Tot. |
880,00 |
520,00 |
520,00 |
238,00 |
158,00 | |
Consumable |
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As per use |