Location: DIBIT2 C1, Floor R, Room 39a
Flow cytometry specialist, FRACTAL
As flow cytometry specialist, Daniela is responsible for supporting researchers in the design and implementation of their experiments. Her experiences include multi-parameter panels set up, high dimensional flow cytometry analysis and small particles detection. She is also involved in the training of new researchers and in solving scientific and technical problems that may occur during their experiments.
Flow cytometry: expertise with Becton Dickinson and Beckman Coulter instruments; QC using the Spherotech Rainbow Calibration Beads and BD Cytometer Setup and Tracking Beads; expertise in multiparameter flow cytometry; CFSE proliferation assay; cell cycle; small particles quantification and analysis; cell sorting with Becton Dickinson instruments.
Analysis software for flow cytometry: Cell Quest; BD DiVa software; Gallios Software; FCS Express.
Cell biology: Isolation and culture of various cell types (PBMC, Wool blood, Murine and Human cell lines, Mesenchymal Stem Cells); cloning by limiting dilution; cryopreservation and thawing; ELISA; CBA (BD) and LEGENDplex (Biolegend) for cytokines quantification;
proliferation assay (CFSE and 3H-thymidine).
Molecular biology: genomic and plasmidic DNA extraction; RNA extraction; RT-PCR; transient and stable transfection; western blot.
Statistics for life sciences: t-test, Analysis of Variance, Correlation, Regression, Survival Analysis (Kaplan-Meier and Cox analysis); use of GraphPad Prism 5.