Location: DIBIT2 A1, Floor 2, Room 23
Senior researcher, Extracellular microenvironment
My group research focuses on the role the extracellular microenvironment play on the onset and progression of diseases in the context of urological pathologies.
From a prospective standpoint, the identification of changes occurring in the biochemical, physical and 3D ultrastructural features of the extracellular matrix associated to diseased tissues will support the identification of new extracellular targets for the delivery of more targeted interventions, as well as the identification of upstream pathways to design innovative therapeutic strategies. Likewise, the characterization of the tissue-associated microbiome represents the second microenvironment studied, which will allow to identifying therapeutic strategies for the engagement prebiotic and/or probiotic therapies in dysbiotic conditions.
The group has strong translational focus, and collaborates with several national and international researchers with different scientific expertise to accomplish an integrated view of the disease.
Alfano M, Ferrarese R, Locatelli I, Ventimiglia E, Ippolito S, Gallina P, Cesana D, Canducci F, Pagliardini L, Viganò P, Clementi M, Nebuloni M, Montorsi F, Salonia A. Testicular microbiome in azoospermic men – First evidence of the impact of an altered microenvironment. Hum Reproduction. 2018 Jul 1;33(7):1212-1217.
Alfano M, Ventimiglia E, Locatelli I, Capogrosso P, Cazzaniga W, Pederzoli F, Frego N, Matloob R, Saccà A, Pagliardini L, Viganò P, Zerbi P, Nebuloni M, Pontillo M, Montorsi F, Salonia A. Anti-mullerian hormone-to-testosterone ratio is predictive of positive sperm retrieval in men with idiopathic non-obstructive azoospermia. Scientific Reports. Dec 2017;7:17638.
Alfano M, Nebuloni M, Allevi R, Zerbi P, Longhi E, Lucianò R, Locatelli I, Pecoraro A, Indrieri M, Speziali C, Doglioni C, Milani P, Montorsi F, Salonia A. Linearized texture of three-dimensional extracellular matrix is mandatory for bladder cancer cell invasion. Scientific Reports. 2016 Oct 25;6:36128.
Cazzaniga W, Nebuloni M, Longhi E, Locatelli I, Allevi R, Lucianò R, Senatore G, Ventimiglia E, Cucchiara V, Genovese L, Montorsi F, Alfano M, Salonia A, Cavarretta I.Human prostate derived extracellular matrix as a model of prostate microenvironment. European Urology Focus. 2016;2(4):400-408.
Alfano M, Canducci F, Nebuloni M, Montorsi F, Salonia A.The interplay of extracellular matrix and microbiome in urothelial bladder cancer. Nature Reviews Urology. 2016 Feb;13(2):77-90.
Nebuloni M, Albarello L, Andolfo A, Magagnotti C, Genovese L, Locatelli I, Tonon G, Longhi E, Zerbi P, Allevi R, Podestà A, Puricelli L, Milani P, Soldarini A, Salonia A, Alfano M. Insight on colorectal carcinoma infiltration by studying perilesional extracellular matrix. Scientific Reports. 2016 Mar 4;6:22522.
Alfano M, Cinque P, Giusti G, Proietti S, Nebuloni M, Danese S, D'Alessio S, Genua M, Portale F, Lo Porto M, Singhal PC, Rastaldi MP, Saleem MA, Mavilio D, Mikulak J. Full-length soluble urokinase plasminogen activator receptor down-modulates nephrin expression in podocytes. Scientific Reports. 2015 Sep 18;5:13647.
Genovese L, Zawada L, Tosoni A, Ferri A, Zerbi P, Nebuloni M, Alfano M. Cellular Localization, Invasion and Turnover Are Differently Influenced by Healthy and Tumor Derived Extracellular Matrix. Tissue Eng Part A. 2014 Jul;20(13-14):2005-1.
Since 2014 Senior researcher, Urological Research Institute, IRCCS Ospedale San Raffaele, Milan, Italy
2014 Teaching qualification as “Associate Professor” in Molecular Biology
2010 Editor for the E-book "Soluble factors mediating innate immune responses to HIV infection". Bentham Science Publishers
2007 Scientific Tutor (code number C0000410, protocol number 00027 January 19, 2007) for two INGENIO projects (sponsored by Regione Lombardia, Milan, Italy; www.ingenio-lombardia.it)
2005-05/2014 Senior researcher, AIDS Immunopathogenesis Unit, IRCCS Ospedale San Raffaele, Milan, Italy
2002-2005 Researcher, AIDS Immunopathogenesis Unit, IRCCS Ospedale San Raffaele, Milan, Italy
2001-to date Supervisor of graduates students, doctorates and post-docs working at the AIDS Immunopathogenesis Unit, IRCCS Ospedale San Raffaele, Milan, Italy
Sept./Dec.-2000 Adjunct Scientist, Laboratory of Cellular and Molecular Biophysics, NICHD, National Institute of Health (NIH), Washington D.C., USA
2000-2001 Post-Doctoral Scientist, AIDS Immunopathogenesis Unit, IRCCS Ospedale San Raffaele, Milan, Italy
1997-2000 Postdoctoral Investigator, Laboratory of Molecular Biology of HIV, The Picower Institute for Medical Research, New York, U.S.A.
1995-1996 Research fellow, AIDS Immunopathogenesis Unit, IRCCS Ospedale San Raffaele, Milan, Italy
1995-1996 Consulting biologist, Experimental Endocrinology Laboratory, Dept. of Pharmacology, Università degli Studi di Milano, Milan, Italy
1992-1995 Research fellow, Laboratory of Biochemistry, IRCCS Ospedale San Raffaele, Milan, Italy
1990-1992 Research internship, Biomedical Engineering Laboratory, Mario Negri Institute for Pharmacological Research, Bergamo, Italy
2001 PhD in Physiopathology, Clinics and Diagnostics of the Immunocompromized Host, Università degli Studi di Milano, Italy
1995 Post-Graduate Course in Scientific Communication, Università degli Studi di Milano, Italy
1993 State License in Biological Sciences, Università degli Studi di Milano, Italy
1992 M.SC. degree in Biological Sciences, Università degli Studi di Milano, Italy