Genetics and cell biology
Protein transport and secretion
Over the last decade, the lab focused on two tightly related problems, the pathophysiology of lymphocyte B to plasma cell development and redox regulation/signaling in the early secretory pathway. Important traits d’union between the two are ERp44, a multifunctional chaperone the group discovered a dozen years ago, which is involved in the assembly and quality control of polymeric IgM and the biogenesis and homeostatic control of the endoplasmic reticulum and secretory organelles.
Cornelius J, Cavarretta I, Pozzi E, Lavorgna G, Locatelli I, Tempio T, Montorsi F, Mattei A, Sitia R, Salonia A, Anelli T. Endoplasmic reticulum oxidoreductase 1 alpha modulates prostate cancer hallmarks. Transl Androl Urol. 2021 Mar;10(3):1110-1120.
Tempio T, Orsi A, Sicari D, Valetti C, Yoboue ED, Anelli T, Sitia R. A virtuous cycle operated by ERp44 and ERGIC-53 guarantees proteostasis in the early secretory compartment. iScience. 2021 Mar 1;24(3):102244.
van Anken E, Bakunts A, Hu CA, Janssens S, Sitia R. Molecular Evaluation of Endoplasmic Reticulum Homeostasis Meets Humoral Immunity.Trends Cell Biol. 2021 Jul;31(7):529-541.
Sicari D, Chatziioannou A, Koutsandreas T, Sitia R, Chevet E. Role of the early secretory pathway in SARS-CoV-2 infection. J Cell Biol. 2020 Sep 7;219(9):e202006005.
Armengaud J, Delaunay-Moisan A, Thuret JY, van Anken E, Acosta-Alvear D, Aragón T, Arias C, Blondel M, Braakman I, Collet JF, Courcol R, Danchin A, Deleuze JF, Lavigne JP, Lucas S, Michiels T, Moore ERB, Nixon-Abell J, Rossello-Mora R, Shi ZL, Siccardi AG, Sitia R, Tillett D, Timmis KN, Toledano MB, van der Sluijs P, Vicenzi E. The importance of naturally attenuated SARS-CoV-2in the fight against COVID-19. Environ Microbiol. 2020 Jun;22(6):1997-2000.
Sitia R, Rubartelli A. Evolution, role in inflammation, and redox control of leaderless secretory proteins. J Biol Chem. 2020 May 29;295(22):7799-7811.
Carmona M, de Cubas L, Bautista E, Moral-Blanch M, Medraño-Fernández I, Sitia R, Boronat S, Ayté J, Hidalgo E. Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity. Nat Commun. 2019 Oct 4;10(1):4526.
